Skills

• Work focus is biomarker discovery for disease (Malaria, Tuberculosis) detection and prediction.
• Validation of kit for qualitative detection of Tuberculosis in biological fluid.
• Antioxidant Assay: DPPH Assay, Nitroblue Tetrazolium Assay, Ferric Reducing Power Assay, ABTS Assay.
• Biochemical Tests: Carbonic Anhydrase Assay, Pancreatic Lipase Assay (Colorimetric and Titrimetric), Amylase Activity Assay, Phospholipase A2 Assay, Catalase Assay, Protease inhibition Assay. Screening of various extracts for Pancreatic Lipase, Phospholipase A2 and Carbonic Anhydrase inhibitory activity and Determination of IC₅₀ value, Specific activity of enzyme.
• Phytochemical estimation.
• Protein Extraction, Estimation, Crystallization, Immobilization and Purification by Ammonium salt precipitation and Acetone precipitation and Characterization by Polyacrylamide Gel Electrophoresis, Detection of Isoelectric point.
• Thin Layer Chromatography.
• Gel Filtration Chromatography.
• Tributyrin Agar Plate method.
• Zymogram for enzyme (Lipase, PLA2).
• Immunology techniques:
• Nanobiotechnology: Biological synthesis of Nanoparticle, Chemical synthesis of Silver Nanoparticle, Synthesis of Cerium Oxide Fluroscent Nanoparticle.
• Microbiology: Antimicrobial Sensitivity Test by Agar well diffusion method, Minimum Inhibitory concentration by Broth Dilution Method.
• Ethanol estimation by potassium dichromate method and by Hygrometer.
• Determination of Arsenic and Fluoride in water.
• Quantification of degradation products of C-terminal telopeptides of type II collagen (CTX-II) in urine
• Supporting staff for M.Sc. Molecular Biology and MSc. Medical Biotechnology.
• Preperation of SOPs of OMICS Research Center.
• Drug Discovery

• Microbial analysis of Raw material, Bulk and finished products.
• Microbial analysis of potable and purified water.
• Sterility testing of finished products.
• Area qualification: i) Settle plate technique (Plate Exposure),   ii) Air Sampling.
• • Identification and maintenance of standard cultures.
  • Documentation in Microbiology department.
  • Maintaining microbiology lab as per Good laboratory practices.
  • Personal hygiene by Swab Test.
  • Calibration of instruments like PH Meter, Weighing Balance, Hot Air Oven, Incubator.

Refrences

M.G.M. Institute of Health Sciences, OMICS Research Centre,  MGMIHS, Kamothe, Navi Mumbai from 1 October, 2016 –26 June,2019 as Junior Research Assistant.     

M.G.M. Hospital, Kalamboli, Navi Mumbai from 1 October, 2016 –26 June,2019 as Junior Research Assistant.

 Alivira Animal Health Ltd., formerly Known as Sequent Scientific Ltd., Ambernath from 28, October 2015 to 30 September, 2016 as Microbiologist Officer.

M.Sc dissertation done at KET'S Scientific Research Centre, Mulund (E) on a research project entitled “Phytochemical Characterisation And In-Vitro Anti-Oxidant, Anti-Inflammatory, Wound Healing and Moisturising Activity of Medicinal Plants” Under the guidance of Dr. Kshitij V. Satardekar.

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