Skills

Human cell line culture, fluorescence microscopy, confocal microscopy, stimulated emission and depletion (STED) microscopy, immunohistochemistry, ELISA, and Metabolic activity assay

Refrences

Project: “Effect of acrolein in comparison to its prodrug cyclophosphamide on human primary endothelial cells in vitro”

• ·       Established endothelial cells cultures
• ·       Evaluated the effect of stress fibers by immunostaining, confocal microscope
• ·       Quantification of nitric oxide, prostacyclin, thromboxane, and cell membrane integrity                                  

Project: “Age related morphology and function of human arterial endothelial cells”

• ·       Established endothelial cell cultures from donors of different age groups
• ·       Evaluated the effect of age on ECM proteins and stress fibers by immunostaining, confocal and STED microscope
• ·       Quantification of ?-galactosidase activity and fluorescent staining with senescence markers, and also cell membrane integrity, nitric oxide, prostacyclin, thromboxane

Time and Spatial Flexiblity

I am open for travel around.

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